What we suggest to start an NMR project...

 1. Expression System/Cell types

                 BL21(DE3)
                HMS174(DE3)
                We typically use pET expression vectors
             

 2. Appropriate Buffers

                 Tris
               Hepes
               Phosphate
               **if you have questions about a buffer, just give us a call**

 3. Sample Conditions
             
                Protein Concentration: We recommend a protein concentation of between 
                   0.2mM - 2mM (less than 0.2mM is possible for some experiments if necessary)
                Sample Volume: Samples need to be 600ul if using a standard NMR tube,
                 or no less than 300ul if using a Shigemi tube
                D2O: The final NMR sample must contain between 5% and 10% D2O
                Salt Concentrations: Try to keep this LOW (<100mM total ionic strength)

 4.    Things   to buy...             
             Sample Tubes:
                          Shigemi
                          Wilmad
                Labeled Isotopes:
                          CIL for 13C-glucose
                          CIL for 15N-ammomium sulfate
                CIL for D20