Our laboratory is interested in the molecular characterization of the mechanism of Ca2+ transport by a group of ATPases associated with intracellular membranes. These ATPases play an important role in control of the cytosolic Ca2+ concentration and, thereby, in regulation of cellular functions by Ca2+. Our experimental approach includes isolation of intracellular membranes; purification of proteins; kinetic measurements of ion fluxes and coupled enzyme reactions; studies of protein structure and function by chemical derivatization and site directed mutagenesis; gene transfer to express recombinant protein and to influence cellular functions.
Suming Hua, Daniele Fabris, and Giuseppe Inesi (1999) "Characterization of Calcium, Nucleotide, Phosphate, and Vanadate Bound States by derivation of sarcoplasmic reticulum ATPase with ThioGlo1." Biophysical Journal,77, 2217-2225.