William R. Randall, Ph.D., Core Leader Produces recombinant adenovirus expressing cardiac proteins to serve the specific aims of the three projects of the PPG. The principal goal of the Molecular Genetics Core is to coordinate, produce, and provide recombinant adenovirus expressing cardiac proteins to serve the specific aims of the three projects of this PPG. Although the emphasis of this core is to maximize the output of adenoviral constructs for the projects, it is also responsible for performing several cloning procedures, when needed, to create the adenovirus constructs. These include creating fusion proteins containing autofluorescent and epitope tags, site directed mutagenesis of cardiac proteins, and producing truncated and chimeric proteins from supplied cDNAs as required by the three projects. The Molecular Genetics Core has produced recombinant adenovirus preparations that express a wide variety of proteins that range from cytoskeletal proteins to kinase and phosphatase subunits expressed in cardiomyocytes. Many of these are being used in two or more projects.
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