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Assistant Professor
 108N. Greene Street, Room 239
Baltimore, Maryland 21201
Phone: (410)706-8904
Fax: (410)706-8297
email: gwils001@umaryland.edu Research Description
In my laboratory, we study cellular mechanisms that regulate the production of many important gene products, including oncoproteins, inflammatory mediators, and lipoprotein receptors. In particular, we focus on protein factors that regulate cytoplasmic mRNA turnover rates, and the signaling systems that may transiently modulate the activity of these factors. Our experimental approaches vary from cell and molecular biology (cultured cell systems, transfection, RNA interference) to biochemical (gel mobility shift, protein-protein and protein-RNA cross-linking), and biophysical systems (fluorescence anisotropy, resonance energy transfer). Some current foci of interest include: - RNA substrate recognition by zinc-finger peptides
- Regulation of protein binding by local higher-order RNA structures
- Regulation of LDL receptor mRNA stability and subcellular localization
MBIC 608 Classroom Powerpoint presentations
Lab personnel:
Jeff Ballin Ph.D. – postdoctoral fellow
Brandy Brewer B.S. – research technician and lab manager Elizabeth Fialcowitz B.S. – graduate student
Sarah Brennan B.S. – graduate student
Noelle Briones M.S. – graduate student
Jun Huang B.S. – graduate student
Recent Publications:
Fialcowitz, E.J., Brewer, B.Y., keenan, B., and Wilson, G.M. (2005) A hairpin-like structure
within an AU-rich mRNA-destabilizing element regulates trans-factor binding selectivity and
mRNA decay kinetics. J. Biol. Chem., in press.
Brewer, B. Y., Malicka, J., Blackshear, P.J., and Wilson, G. M. (2004) RNA sequence elements
required for high affinity binding by the zinc finger domain of tristetraprolin: Conformational
changes coupled to the bipartite nature of AU-rich mRNA-destabilizing motifs. J. Biol. Chem.,
279, 27870-27877 Wilson, G.M., Lu, J., Sutphen, K., Suarez, Y., Sinha, S., Brewer, B., Villanueva-Feliciano, E.C.,
Ysla, R.M., Charles, S., and Brewer, G. (2003) Phosphorylation of p40AUF1 regulates binding to
A+U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein
structure. J. Biol. Chem., 278, 33039-33048 Wilson, G.M., Lu, J., Sutphen, K., Sun, Y., Huynh, Y., and Brewer, G. (2003) Regulation of
A+U-rich element-directed mRNA turnover involving reversible phosphorylation of AUF1. J.
Biol. Chem., 278, 33029-33038 |
