EDUCATION
1973     Ph.D. in Biochemistry, University of Illinois, Urbana, Illinois
1971     M.S. in Biochemistry, University of Illinois, Urbana, Illinois
1970     B.A. in Chemistry, Maxima Cum Laude, LaSalle College, Philadelphia, Pennsylvania
1969    Temple University, Philadelphia, Pennsylvania (Additional mathematics courses, Summer)
1968     Drexel Institute of Technology, Philadelphia, Pennsylvania (Additional mathematics courses, Summer)

PROFESSIONAL EXPERIENCE
1984-present    Professor, Department of Biochemistry and  Molecular Biology, University of Maryland at
                       Baltimore, School of Medicine
1993-present    Adjunct Professor, Electrical Engineering, University of Maryland, Baltimore County
1988-present    Senior Staff Scientist, Medical Biotechnology Center, University of Maryland at Baltimore
1988-present    Director, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine,
                       Department of Biochemistry and Molecular Biology
1989-1993        Head, Molecular Graphics Facility, University of Maryland School of Medicine
1980-1984        Associate Professor, Department of Biological Chemistry,  University of Maryland at Baltimore,
                       School of Medicine
1975-1980        Assistant Professor of Biochemistry, Department of Biochemistry and Gray Freshwater
                       Biological Institute, University of Minnesota. Promoted to Associate Professor with tenure,
                       effective July 1980
1974-1975        Research Associate, Freshwater Biological Institute, University of Minnesota
1973-1974        Postdoctoral Fellow, Oxford University, Department of Biochemistry, Oxford, England, Nuclear
                       Magnetic Resonance

RESEARCH DESCRIPTION 
This laboratory is involved in the use and development of fluorescence spectroscopic methods. Our present research projects to advance fluorescence technology include two-photon induced fluorescence and the use of light to modify the excited state populations.

The continued development of advanced fluorescence methods is performed at the Center for Fluorescence Spec-tro-sco-py, which is a National Resource of the NIH. This cen--ter provides state-of-the-art time and frequency-domain measurements to users from this campus and from other institutions in this country as well as other countries. Our basic science projects include the use of fluorescence to study:

• Conformational distributions of proteins, nucleic acids, and transfer RNA.
• Dynamics of proteins and membranes.
• Transient effects in diffusion in solutions, and in proteins and membranes.
• Synthesis of fluorescent sensor molecules.
• Development of fluorescent lifetime imaging microscopy.
• Multi-photon excitation.

ASSOCIATED SITES:  Center for Fluorescence Spectroscopy

                                  Principles of Fluorescence Spectroscopy

SELECTED PUBLICATION:
A Wavelength-Ratiometric Fluoride-Sensitive Probe Based on the Quinolinium Nucleus and Boronic Acid Moiety, Badugu, R., Lakowicz,J. R., and Geddes, C. D. (2005). Sensors Actuators B 104:103-110.

Fluorescence Enhancement of Fluorophores Tethered to Different Sized Silver colloids Deposited on Glass Substrate, Lukomska, J., Malicka, J., Gryczynski, I., Leonenko, Z., and Lakowicz, J. R. (2005). Biopolymers 77:31-37.

Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films, Lukomska, J., Gryczynski, I., Malicka, J., Makowiecz, S., Lakowicz, J., and Gryczinski, Z. (2005). Biochemical and Biophysical Research Communications, 328: 78-84.

Nanogold Plasmon Resonance-Based Glucose Sensing. 2. Wavelength- Ratiometirc Resonance Light Scattering, Aslan, K., Lakowicz, J., and Geddes C.D. (2005). Analytical Chemistry, 77: 2007-2014.

Metal-enhanced fluorescence: an emerging tool in biotechnology, Aslan, K., Gryczinski, I., Malicka, J., Matveeva, E. Lakowicz, J. ,and  Geddes, C.D. (2005) Current Opinion in Biotechnology, 16:1-8

Fast and Slow Deposition of Silver Nanorods on Planar Surfaces: Application to Metal-Enhanced Fluorescence, Aslan, K., Leonenko, Z., Lakowicz. J., and  Geddes, C.D. (2005).  J. Phys. Chemistry, 109: 3157-3162.

Enhanced Fluorescence cyanide detection at physiologically lethal levels: Reduced ICT-based signal transduction, Badugu, R., Lakowicz, J., and Geddes, C.D. (2005).  J. Am. Chem.Society, 127: 3635-3641.

Rapid depositing of triangular silver nanoplates on planar surfaces: Application to metal-enhanced fluorescence, Aslan, K., Lakowicz, J., and Geddes, C.D. (2005). J. Phys. Chem. B, 109: 6247-6251.

A glucose-sensing contact lens: from bench top to patient, Badugu, R., Lakowicz, J., and Geddes, C.D. (2005). Current Opinion in Biotechnology, 16: 100-107.  

Fluorescence sensors for monosaccharides based on the 6-methylquinolinium nucleus and boronic acid moiety: potential application to ophthalmic diagnostics, Badugu, R., Lakowicz,J., and Geddes, C.D. (2005). Talanta, 65: 762-768.

Enhanced lanthanide luminescence using silver nanostructures: Opportunities for a new class of probes with exceptional spectral characteristics, Wu, M., Lakowicz, J., and Geddes, C.D.(2005) Journal of Fluorescence, 15: 53-59.

Enhanced ratiometric pH sensing using SNAFL-2 on silver island films- metal-enhanced fluorescence sensing, Aslan, K., Lakowicz, J., Szmacinski, H., and Geddes, C.D. (2005). Journal of Fluorescence, 15: 37-40.

Radiative decay engineering 5: metal-enhanced fluorescence and plasmon emission, Lakowicz, J. (2005). Analytical Biochemistry, 337: 171-194.

Surface-plasmon-coupled emission of quantum dots, Gryczinski, I., Malicka, J., Jiang, W., Fischer, H., Chan, W., Gryczinski, Z., Grudzinski, W., and  Lakowicz, J. (2005). J. Phys.Chem. B, 109: 1088-1093.

 Wavelength-ratiometric near-physiological pH sensors based on 6-aminoquinolinium boronic acid probes, Badugu, R., Lakowicz, J. R., and Geddes, C. D. (2005). Talanta 66:569-574.

Enhanced luminescence of phenyl-phenanthridine dye on aggregated small silver nanoparticles, Zhang, J. and Lakowicz, J. R. (2005). J. Phys. Chem. B, 109:8701-8706.

Boronic acid fluorescent sensors for monosaccharide signaling based on the 6-methoxyquinolinium heterocyclic nucleus: progress toward noninvasive and continuous glucose monitoring, Badugu, R., Lakowicz, J., and Geddes, C.D. (2005). Bioorganic and Medicinal Chemistry, 13: 113-119.

Metal-enhanced Fluoroimmunoassay on a Silver Film by Vapor Deposition, Zhang, J., Matveeva, E., Gryczynski, I., Leonenko, Z., and Lakowicz, J. R. (2005). J. Phys. Chem. B 109:7969-7975.

Surface-enhanced Fluorescence of Fluorescein-Labeled Oligonucleotides Capped on Silver Nanoparticles, Zhang, J., Malicka, J., Gryczynski, I., and Lakowicz, J. R. (2005). J. Phys. Chem. B. 109:7643-7648.

Metal-enhanced fluorescence from plastic substrates, Aslan, K., Badugu, R., Lakowicz, J. R., and Geddes, C. (2005). J. Fluorescence 15(2):99-104.

Anion sensing using quinolinium based boronic acid probes, Badugu, R., Lakowicz, J. R., and Geddes, C. D. (2005). Current Analytical Chemistry 1:157-170.

Metal-enhanced fluorescence using anisotropic silver nanostructures: critical progress to date, Aslan, K., Lakowicz, J. R., and Geddes, C. D. (2005). Anal. Bioanal. Chem. 382:926-933.

Myoglobin immunoassay based on metal particle-enhanced fluorescence, Matveeva, E. G., Gryczynski, Z., and Lakowicz, J. R. (2005). J. Immunol. Methods 302:26-35.

Angular-ratiometric plasmon-resonance based light scattering for bioaffinity sensing, Aslan, K., Holley, P., Davies, L., Lakowicz, J. R., and Geddes, C. D. (2005). J. Am. Chem. Soc. 127:12115-12121.

Plasmonic technology: Novel approach to ultrasensitive immunoassays, Lakowicz, J. R., Malicka, J., Matveeva, E., Gryczynski, I., and Gryczynski, Z. (2005). Clin. Chem. 51:1914-1922.