| Professor
108 N. Greene St.
Baltimore, MD 21201
Phone: 410-706-3510
Fax: 410-706-8297
email: lblack@umaryland.edu
EDUCATION
B.S. - Biochemistry, The University of Chicago (Honors, Phi Beta
Kappa)
Ph.D. - Biochemistry, Department of Biochemistry,
Stanford University School of Medicine Post Doctoral - Institute of Molecular Biology,
University of Geneva, Switzerland,
Post Doctoral Fellow - Jane Coffin Childs Foundation
Swiss National Science Foundation
PROFESSIONAL EXPERIENCE
1971-1973 Asst. Prof., Dept. Biol. Chem., School of Medicine, University of Maryland.
1974 Visiting Scientist, Microbiology Dept. Biozentrum Basel University, Basel, Switzerland.
1973-1982 Assoc. Prof., Biol. Chemistry, School of Medicine, University of Maryland.
1983-present Professor, Dept. of Biochemistry & Mol. Biology, School of Medicine, University of Maryland.
RESEARCH DESCRIPTION
1) My long standing interest is the mechanism of viral nucleic acid packaging: Nucleic acid packaging into viral precursors is highly conserved biologically among phages and viruses. Although translocation is known to result from the ATPase activity of a packaging enzyme that interacts with a dodecameric portal vertex of the procapsid, the energetic mechanism remains to be established. We have studied intensively the phage T4 proteins (portal dodecamer and two subunit packaging enzyme) that package DNA.
2) Phage Display and encapsidation: We have developed the two protein phage T4 SOC and HOC capsid display system to reveal protein interactions important for viral packaging. These proteins enable display of full length biologically and immunologically active proteins for binding studies and development of vaccines. A phage T4 capsid targeting sequence also allows virtually any protein to be encapsidated with the DNA.
3) Phage exclusion and anti-exclusion mechanisms: A novel type IV modification dependent restriction enzyme that targets glycosylated hydroxymethyl cytosine modified DNAs has been isolated and characterized from pathogenic E. coli CT596 prophage exclusion genes ibegs and gibeg. The T-evens have evolved a diverse family of capsid-targeted internal proteins injected into the host with the DNA to shield the diverse sugar modifications of their hydroxymethyl cytosine residues from the IBEG family of MDS enzymes.
LAB PERSONNEL:
Julienne Mullaney, Research Associate
Dalin Rifat, Research Associate
Guihong Peng, Post Doctoral
Former UMB graduate students:
Ken Abremski
Chulai Hsiao
Duu-Gong Wu
Yi-Ren Hong
Herbert Wu
Jinny Lin
Richard Baumann
Catherine Bair
SELECTED PUBLICATIONS in research areas 1-3 above:
Area 1 of Research
Rao, VB, Black, LW (2005) DNA packaging in bacteriophage T4. in Viral Packaging Machines, (ed. Catalano C), Kluwer Academic/Plenum. PDF
Fokine A, Leiman PG, Shneider MM, Ahvazi B, Boeshans KM, Steven AC, Black LW, Mesyanzhinov VV, and Rossmann MG (2005), Structural and functional similarities between the capsid proteins of bacteriophages T4 and HK97 point to a common ancestry. PNAS in press.
Richard G. Baumann and Lindsay W. Black (2003) Isolation and Characterization of T4 Bacteriophage gp17 Terminase,
a Large Subunit Multimer with Enhanced ATPase Activity. J. Biol. Chem. 278, 4618-4627.
Black, L.W., (1989). DNA Packaging in dsDNA Bacteriophages. Annu. Rev. Microbiol. 43: 267-292 Area 2 of Research Naglis Malys, Dau-Yin Chang, R.G. Baumann, Dongmei Xie, and Lindsay W. Black* (2002)A bipartite bacteriophage T4 SOC and HOC randomized peptide display library: detection and analysis of phage T4 terminase (gp17) and late sigma factor (gp55) interaction. J. Mol. Biol. 319, 289-304. PDF
Mullaney, J.M., Thompson, R.B., Gryczynski, Z.K. and Black, L.W. (2000). Green fluorescent protein as a probe of rotational mobility within bacteriophage T4. Journal of Virological Methods 88, 35-40.
Area 3 of Research
Catherine Bair, Dalin Rifat, and Lindsay Black (2005) Evolution of the Myoviridae internal protein I locus genes in response to host type IV modification dependent restriction enzymes, Phage/Virus Assembly, Colorado, 2005
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